A novel and with high saline and high thermo stable enzyme??s gene encoding a ??-1,4-endoxylanase, named xyn6, was successfully cloned from Bacillus sp. and was heteroexpressed in Escherichia coli BL21 (DE3).The recombinant XYN6 showed an maximal activity at 60??, pH 6.0. It also showed an optimal activity at 15-20% NaCl, and substantial activity remained at 30% NaCl. The xylanase XYN6 showed high activity under saline conditions, and shown to have high industrial potential for various industrial processes such as wastewater treatment and bioethanol production.
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