Scenedesmus sp. may be a potential strain for integrating piggery wastewater treatment with algae biodiesel production. Results from the CCD tests show that consideration of the experimental range, the most important factor for lipid productivity and nutrient removal is incubation time. The highest lipid productivity (25.23 mg/L.d) and nutrient removal were obtained at the following conditions: piggery wastewater content (50 %), biomass concentration (OD > 0.3), and incubation time (5-7 days). The mixotrophic cultivation not only produced biomass, but also could assimilate up to 78.56 % total nitrogen, 70.38 % total phosphorus, and 80.91 % COD from piggery wastewater, respectively.