Monoraphidium sp. is exceedingly rich in lipids and oils, which can be converted into biodiesel. The FAMEs yield increased with decreasing moisture of wet microalgae biomass. However, no significant difference in FAMEs yield affected by moisture of wet microalgae biomass was found when reaction with suitable catalyst amount was adopted. The highest FAMEs yield of wet microalgae biomass was obtained at a 25 mL acid catalyst amount containing 0.5 % NaOH/MeOH and 2 % H2SO4/MeOH respectively. Furthermore, it was observed that alkali-acid-catalyzed transesterification led to a higher FAMEs yield than that obtained only alkali or acid catalyzed transesterification.