This innovative work exhibits 3D stereoscopic images with interactive features. By using augmented reality technology, the interaction of the virtual fish collection and the user turns to be real. By touching the virtual fish, they will run away or turn around. Two clicks at the virtual fish, a pop-up of the corresponding profile will come out. Moreover, the conjunction between the virtual image of the detected marker or gesture and the multi-layered virtual fish collection in the image field presents a new experience of depth perception to the user. There is no need to wear glasses just to have a 3D stereoscopic experience. The hardware requirements are affordable and easy to maintain, and the content of the fish collection can be very adaptable to one??s preference. From personal to public, the 3D stereoscopic images with interactive features can be an innovative navigator for users and bring them a new tour full of intellectual and emotional.